Invitrogen

NGAL Monoclonal Antibody (29)

NGAL Antibody in Immunocytochemistry (ICC/IF)

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NGAL Antibody (ABS 043-29-02) in ICC/IF

Immunofluorescence analysis of NGAL was performed using 70% confluent log phase RAW 264.7 treated with Thapsigargin (250 nm, 48hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NGAL Monoclonal Antibody (29) (Product # ABS 043-29-02) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody... View More

NGAL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NGAL Antibody — Detection of altered subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis using NGAL Monoclonal Antibody (29) (Product # ABS 043-29-02), shows an increase in expression of NGAL in RAW 264.7 upon Thapsigargin treatment. {TM}

产品信息

ABS 043-29-02

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:1,000

免疫细胞化学 (ICC/IF)

5 µg/mL

酶联免疫吸附实验 (ELISA)

1:4,000

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

抗原

Recombinant mouse NGAL adsorbed onto aluminum hydroxide gel.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.92 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_2136588

产品详细信息

ABS 043-29-02 binds free NGAL in mouse serum.

This antibody will not cross react with human, pig, dog, or rat.

ABS 043-29-02 may be used in Western blot and ELISA applications.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

靶标信息

NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Zebrafish GSDMEb Cleavage-Gated Pyroptosis Drives Septic Acute Kidney Injury In Vivo.

Journal of immunology (Baltimore, Md. : 1950)

Wang Z,Gu Z,Hou Q,Chen W,Mu D,Zhang Y,Liu Q,Liu Z,Yang D

ABS 043-29-02 was used in Western Blot to use a zebrafish crispant in vivo analysis model to investigate LPS-induced septic shock.

Wed Apr 01 00:00:00 UTC 2020

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

ABS 043-29-02 was used in Western Blot to show that pFUS may be a strategy for enhancing the therapeutic efficacy of MSC-derived EV treatment for the treatment of AKI.

Mon Sep 14 00:00:00 UTC 2020

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

ABS 043-29-02 was used in Western Blot to show that pFUS may be a strategy for enhancing the therapeutic efficacy of MSC-derived EV treatment for the treatment of AKI.

Mon Sep 14 00:00:00 UTC 2020

生物信息学

蛋白别名: 24p3; HGNC:6526; Lipocalin-2; Lipocalin2; Neutrophil gelatinase-associated lipocalin; NGAL; Oncogene 24p3; OTTHUMP00000022240; p25; RP23-161B9.11; secreted inducible protein 24; Siderocalin LCN2; SV-40-induced 24P3 protein; uterocalin

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基因别名: 24p3; AW212229; Lcn2; Sip24

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UniProt ID: (Mouse) P11672

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Entrez Gene ID: (Mouse) 16819

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功能

protease binding transporter activity iron ion binding protein binding small molecule binding protein homodimerization activity transfer/carrier protein

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